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JoVE Journal Neuroscience
An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
 

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

Article DOI: 10.3791/57038-v • 09:35 min • February 26th, 2018
February 26th, 2018 •
1,2, 1, 1, 1
1Institute of Neurology (Edinger Institute), Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology, Goethe University Hospital

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Summary
Automatic Translation

Here we present a mouse brain vascular permeability assay using intraperitoneal injection of fluorescent tracers followed by perfusion that is applicable to animal models of blood-brain barrier dysfunction. One hemi-brain is used for assessing permeability quantitatively and the other for tracer visualization/immunostaining. The procedure takes 5 - 6 h for 10 mice.

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Keywords: In Vivo Blood-brain Barrier Permeability Fluorescent Tracers Mice Neurological Disorders Brain Edema Blood-brain Barrier Dysfunction Cardiac Perfusion Fluorometry Fluorescence Microscopy
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