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JoVE Journal Neuroscience
Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain
 

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Article DOI: 10.3791/63998-v • 09:44 min • May 20th, 2022
May 20th, 2022 •
1,2, 2, 3, 1,2
1Graduate School of Biomedical Science and Engineering, University of Maine, 2Department of Neurosurgery, Ohio State University Wexner Medical Center, 3Boston Children’s Hospital, Harvard Medical School

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Summary
Automatic Translation

The ability to permanently mark stem cells and their progeny with a fluorophore using an inducible transgenic lineage tracing mouse line allows for spatial and temporal analysis of activation, proliferation, migration, and/or differentiation in vivo. Lineage tracing can reveal novel information about lineage commitment, response to intervention(s), and multipotency.

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Keywords: Lineage Tracing Inducible Fluorescent Labeling Adult Mouse Brain Stem Cells Transgenic Immunostaining Immunofluorescence Antigen Retrieval Triton X-100 Tween-20 Tyrogen Black Alexa Fluor GFP Brain Plasticity Neurodegenerative Diseases Aging
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