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Immunology and Infection
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Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
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Immunology and Infection
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JoVE Journal Immunology and Infection
Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

DOI:
10.3791/56444-v

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12:57 min

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November 16, 2017

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, ,
1Department of Food, Bioprocessing, and Nutrition Sciences,North Carolina State University, 2Department of Chemical and Biomolecular Engineering,North Carolina State University

Chapters

  • 00:05Title
  • 00:49Plate-based Binding Assay for Evaluating Loss of Higher Order Norovirus Capsid Structure
  • 04:44Dynamic Light Scattering (DLS) Detecting Virus Aggregation After Heat Treatment
  • 06:32DLS for Detecting Virus Aggregation in Real Time
  • 07:29Transmission Electron Microscopy (TEM) Sample Preparation
  • 09:34Results: Reduction of Human Norovirus Capsid Stability After Heating
  • 11:36Conclusion

Summary

Automatic Translation

Automatic Translation

Routine detection methods utilizing viral genome amplification are limited by their inability to discriminate infectious from non-infectious particles. The purpose of this article is to provide detailed protocols for alternative methods to aid in discrimination of infectious norovirus particles using aptamer binding, dynamic light scattering, and transmission electron microscopy.

Tags

Viral CapsidStructural IntegrityIn Vitro MethodsNorovirusViral InactivationCapsid ProteinHeat TreatmentAptamerELISA

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