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用点法分析细胞板的迁移
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Using the Dot Assay to Analyze Migration of Cell Sheets
DOI:

09:42 min

December 05, 2017

Chapters

  • 00:05Title
  • 00:47Coating Dishes (Day 1)
  • 01:55Preparing Cell Suspension for Dot Assay (Day 2)
  • 03:11Plating Cells
  • 04:22Stimulating Cells (Day 3)
  • 05:19Hematoxylin-Eosin Staining to Measure Colony Growth (Days 4-7)
  • 06:06Time Lapse Microscopy
  • 07:12Results: Analysis of Migration of Cells Sheets Using the Dot Assay
  • 08:55Conclusion

Summary

Automatic Translation

细胞迁移是发展, 组织维护和修复, 和肿瘤发生的关键, 并由生长因子, 趋化因子, 和细胞因子调节。本协议描述的点法, 一个二维, 无约束的迁移分析, 以评估微提示的反应, 连接的, 有凝聚力的细胞表移表型。

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