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Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle
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Biochemistry
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JoVE Journal Biochemistry
Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

DOI:
10.3791/56504-v

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09:30 min

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November 06, 2017

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, , ,
1Department of Functional Genomics and Cancer,Institut de Génétique et de Biologie Moléculaire et Cellulaire

Chapters

  • 00:05Title
  • 00:59Isolation of Muscle Tissue and Tissue Lysis
  • 02:06Fixation and Nuclei Preparation
  • 04:04Assessing the Chromatin Fragment Size Following Sonication
  • 06:46Results: Purification of Nuclei from Adult Mouse Muscle
  • 08:09Conclusion

Summary

Automatic Translation

Automatic Translation

A novel protocol for the preparation of chromatin from adult mouse skeletal muscle adapted to the study of gene regulation in muscle fibers by chromatin immunoprecipitation is presented.

Tags

Keywords: Chromatin ImmunoprecipitationMouse Skeletal MuscleGene RegulationChIP-grade ChromatinFormaldehyde FixationGlycine QuenchingNuclei IsolationCell Strainer FiltrationCentrifugation

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