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改进的小鼠骨骼肌染色质沉淀的协议

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Please note that all translations are automatically generated. Click here for the English version.

改进的小鼠骨骼肌染色质沉淀的协议

DOI:

10.3791/56504-v

•

09:30 min

•

November 06, 2017

•

Shilpy Joshi, Vanessa Ueberschlag-Pitiot, Daniel Metzger, Irwin Davidson

¹Department of Functional Genomics and Cancer,Institut de Génétique et de Biologie Moléculaire et Cellulaire

Chapters

00:05Title
00:59Isolation of Muscle Tissue and Tissue Lysis
02:06Fixation and Nuclei Preparation
04:04Assessing the Chromatin Fragment Size Following Sonication
06:46Results: Purification of Nuclei from Adult Mouse Muscle
08:09Conclusion

Summary

Automatic Translation

本文介绍了一种新的制备成年小鼠骨骼肌染色质的方法, 该方法适合于用染色质沉淀研究肌纤维的基因调控。

Tags

Chromatin Immunoprecipitation Mouse Skeletal Muscle Gene Regulation ChIP-grade Chromatin Formaldehyde Fixation Glycine Quenching Nuclei Isolation Cell Strainer Filtration Centrifugation

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