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Immunology and Infection

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JoVE Journal Immunology and Infection
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
 

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Article DOI: 10.3791/56508-v • 13:14 min • February 6th, 2018
February 6th, 2018 •
1,2,3, 1,2,3, 1,2, 1,2
1CRCINA, Inserm, CNRS, Université d'Angers, Université de Nantes, 2LabEx IGO, "Immunotherapy, Graft, Oncology", 3CHU Nantes, France

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Summary
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We describe a method for the production of human antibodies specific for an antigen of interest, starting from rare B cells circulating in human blood. Generation of these natural antibodies is efficient and rapid, and the antibodies obtained can discriminate between highly related antigens.

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Keywords: Human Monoclonal Antibodies Antigen-specific B Cells Blood Discriminative Antibodies Immunotherapy B Lymphocytes Density Gradient Mononuclear Cells Tetramers Magnetic Microbeads Cell Separation
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