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完整、全小鼠乳腺的分离及细胞外基质表达及腺体形态学分析

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JoVE Journal Biology

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

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完整、全小鼠乳腺的分离及细胞外基质表达及腺体形态学分析

DOI:

10.3791/56512-v

•

12:49 min

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October 30, 2017

•

Christopher Thompson, Katherine Keck, Abigail Hielscher

¹Department of Biochemistry and Molecular Biology,University of Nebraska Medical Center, ²Department of Biomedical Sciences,GA-PCOM

Chapters

00:05Title
00:57Sample Procurement and Processing
02:29Immunohistochemistry
05:02Hematoxylin and Eosin Staining
06:19Ductal Analysis
08:33Immunohistochemictry Analysis
09:43Results: Whole Mouse Mammary Gland Isolation and Analysis
11:23Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个完整的, 完整的小鼠乳腺分离的协议, 以研究细胞外基质 (ECM) 的表达和导管形态学。小鼠 #4 腹腺从8-10 周大的雌性产妇小鼠中提取, 固定在中性缓冲福尔马林中, 用免疫组织化学方法对 ECM 蛋白进行切片和染色。

Tags

Keywords: Mammary Gland Whole Mount Extracellular Matrix Gland Morphology Tumor Biology Stromal Remodeling Gene Expression Protein Expression Ductal Architecture Nulliparous Mice Formalin Fixation Paraffin Embedding Antigen Retrieval Immunohistochemistry

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