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饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡
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Immunology and Infection
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JoVE Journal Immunology and Infection
Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
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饱和脂肪酸诱导神经酰胺相关巨噬细胞死亡

DOI:
10.3791/56535-v

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08:26 min

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October 31, 2017

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, , ,
1Department of Microbiology and Immunology,University of Louisville

Chapters

  • 00:05Title
  • 00:59Preparation of Mouse Bone Marrow Derived Macrophages (BMDMs) and BSA-fatty Acid Conjugate
  • 04:31Saturated Fatty Acids Induce Cell Death of Mouse Bone Marrow Derived Macrophages
  • 06:04Intracellular Ceramide Staining
  • 07:03Results: Fatty Acid-induced Macrophage Cell Death and Ceramide Production
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

本文阐述了一种从骨髓细胞中提取小鼠原发巨噬蛋白的方法, 并提出了制备 BSA-脂肪酸共轭物的简便方法。然后我们证明饱和脂肪酸能诱导巨噬细胞死亡, 这种细胞死亡与神经酰胺水平的细胞积累呈正相关。

Tags

Keywords: Saturated Fatty AcidsMacrophagesCeramidesCell DeathMetabolic InflammationBSA Fatty Acid ConjugatesBone Marrow CellsRed Blood Cell LysisRPMI 1640 MediumMacrophage DifferentiationM-CSF

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