Journal
/
/
En enkel fluorescens-baserade Reporter analysen identifiera cellulära komponenter krävs för Ricin Toxin en kedja (RTA) handel med jäst
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain (RTA) Trafficking in Yeast
DOI:

09:03 min

December 15, 2017

,

Chapters

  • 00:05Title
  • 00:46Heterologous Expression of His-tagged RTA in E. coli
  • 02:12Purification of His-tagged RTA via Affinity Chromatography
  • 04:21Yeast Transformation and Cell Wall Removal
  • 05:28GFP Reporter Assay Measurement in 96-well Plates
  • 06:57Results: Assay to Identify Cellular Components Required for RTA Trafficking in Yeast
  • 08:30Conclusion

Summary

Automatic Translation

I manuskript beskriver vi användning av en jäst-baserade fluorescens reporter analysen identifiera cellulära komponenter inblandade i människohandel och döda processer av den cytotoxiska en delenhet av den växt giftet ricin (RTA).

Related Videos

Read Article