Journal
/
Biology
/
The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects

The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects

DOI:
10.3791/56619-v

•

07:03 min

•

December 20, 2017

•

, , , , , , ,
1Department of Biological Sciences,Kent State University at Stark, 2Department of Mathematical Sciences,Clemson University, 3Liquid Crystal Institute,Kent State University, 4Brookhaven National Laboratory

Chapters

  • 00:05Title
  • 00:38Insect Species, Preparation of Solutions, and Feeding Station Setup
  • 01:43Feeding Protocol
  • 03:05Dissections
  • 04:31Testing for Ingested Nanoparticles
  • 05:24Results: A Relationship Between Feeding Pore Size and Feeding Conduit Size
  • 06:28Conclusion

Summary

Automatic Translation

Automatic Translation

Fluid-feeding insects have the ability to acquire minute quantities of liquids from porous surfaces. This protocol describes a method to directly determine the ability for insects to ingest liquids from porous surfaces using feeding solutions with fluorescent, magnetic nanoparticles.

Tags

Fluorescent Magnetic NanoparticlesFluid-feeding InsectsInsect Mouth PartsInsect Feeding MechanismsBlue Bottle FliesHouse FliesCabbage ButterfliesSucrose SolutionFeeding StationNylon Net FiltersMembrane FiltersMicroscope SlidesManipulatorFeeding StageFilter PaperInsect Pin

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56619?article_type=v

Choice and No-Choice Assays for Testing the Resistance of A. thaliana to Chewing Insects
check_url/56619?article_type=v

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging
check_url/56619?article_type=v

Counting and Determining the Viability of Cultured Cells
check_url/56619?article_type=v

Fluorescent Labeling of Drosophila Heart Structures
check_url/56619?article_type=v

Purification of the M. magneticum Strain AMB-1 Magnetosome Associated Protein MamAΔ41
check_url/56619?article_type=v

Determining the Contribution of the Energy Systems During Exercise
check_url/56619?article_type=v

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
check_url/56619?article_type=v

Methods for the Extraction of Endosymbionts from the Whitefly Bemisia tabaci
check_url/56619?article_type=v

Synthesis of 68Ga Core-doped Iron Oxide Nanoparticles for Dual Positron Emission Tomography /(T1)Magnetic Resonance Imaging
check_url/56619?article_type=v

High-Throughput Assays of Critical Thermal Limits in Insects

Read Article