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Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist
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Immunology and Infection
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JoVE Journal Immunology and Infection
Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

DOI:
10.3791/56655-v

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07:48 min

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April 25, 2018

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,
1Immunology Program,Memorial Sloan-Kettering Cancer Center, 2Weill-Cornell Graduate School of Medical Sciences

Chapters

  • 00:05Title
  • 00:44Preparation of Stimulatory Glass Surfaces
  • 02:48Image Acquisition
  • 04:18Analysis of Fluorescence Intensity
  • 05:10Analysis of Distance
  • 05:58Results: TCR-induced DAG Accumulation and Centrosome Reorientation in 5C.C7 T Cells
  • 07:09Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes an imaging-based method to activate T lymphocytes using photoactivatable peptide-MHC, enabling precise spatiotemporal control of T cell activation.

Tags

Keywords: Photoactivatable Peptide MHCT Cell ActivationSpatial-temporal ControlT Cell SignalingTIRF MicroscopyCD4+ T CellsCD8+ T CellsBiotinylated LigandsStreptavidinBio-PLL

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