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Biology
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酸性細胞外 pH 培養系の確立
JoVE Journal
Biology
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JoVE Journal
Biology
Establishment of an Extracellular Acidic pH Culture System
Please note that all translations are automatically generated.
Click here for the English version.
酸性細胞外 pH 培養系の確立
DOI:
10.3791/56660-v
•
09:41 min
•
November 19, 2017
•
Ayano Kondo
,
Tsuyoshi Osawa
1
Innovative Technology Laboratories
,
Kyowa Hakko Kirin Co., Ltd
,
2
Laboratory for Systems Biology and Medicine, RCAST
,
The University of Tokyo
Chapters
00:05
Title
00:57
Preparation of Acidic Culture Medium
02:50
Harvesting and Treating Cells
04:31
RNA Isolation
06:30
cDNA Synthesis and Analysis
08:01
Results: Transcriptional Upregulation of Acidic responsive Genes in Response to Lactic Acidosis and HCl-mediated Acidosis
08:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
酸性腫瘍微小環境は、腫瘍の進行に重要な役割を果たしています。がん細胞の
in vitro で
の酸性細胞外 pH の効果を評価するためには、単純な酸性文化体制を確立しました。
Tags
Extracellular Acidic PH
Tumor Microenvironment
Cancer Progression
Malignancy
Acidosis
DMEM
Sodium Bicarbonate
L-glutamine
Penicillin-streptomycin
Fetal Bovine Serum
Lactate
HCl
PH Measurement
Cell Culture
PBS
Trypsin
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