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Journal
/
Developmental Biology
/
免疫荧光标记的微管和中心蛋白在
体内
肠道组织和 3D
体外
肠道 Organoids
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in
Ex Vivo
Intestinal Tissue and 3D
In Vitro
Intestinal Organoids
Please note that all translations are automatically generated.
Click here for the English version.
免疫荧光标记的微管和中心蛋白在
体内
肠道组织和 3D
体外
肠道 Organoids
DOI:
10.3791/56662-v
•
09:51 min
•
December 13, 2017
•
Deborah A. Goldspink
,
Zoe J. Matthews
,
Elizabeth K. Lund
,
Tom Wileman
,
Mette M. Mogensen
1
School of Biological Sciences
,
University of East Anglia
,
2
Norwich Medical School
,
University of East Anglia
Chapters
00:05
Title
01:41
Isolation of Intestinal Organoids from Basement Matrix Domes in 24-well Plates
03:20
Fixation of Isolated Organoids
04:22
Blocking, and Primary and Secondary Antibody Incubation
06:41
Mounting Isolated Organoids
07:39
Results: Isolation and Immunolabeling of Intestinal Organoids
08:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了从
体内
组织和
体外
基底基质 organoids 分离肠道3D 结构的协议, 并详细介绍了针对微管免疫标记优化的不同固定和染色方案,中心, 和连接蛋白以及细胞标记包括干细胞蛋白 Lgr5。
Tags
3D Intestinal Organoids
Immunofluorescent Labeling
Microtubules
Centrosomal Proteins
Fixation
Isolation
Immunostaining
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