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Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
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Genetics
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JoVE Journal Genetics
Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

DOI:
10.3791/56736-v

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10:39 min

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February 26, 2018

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, ,
1Department of Biochemistry and Molecular Biology,Johns Hopkins University Bloomberg School of Public Health

Chapters

  • 00:04Title
  • 00:59Harvesting Fetal or Neonatal Ovaries
  • 03:55Metaphase I (MI) or Metaphase II (MII) Oocyte Collection
  • 05:17Oocyte Denuding and Zona Pellucida Removal
  • 07:05MI and MII Oocyte Chromatin Spreads
  • 08:08Results: Crossover Formation in C57B/6J Oocytes
  • 09:52Conclusion

Summary

Automatic Translation

Automatic Translation

Oogenesis in mammals is known to be error-prone, particularly due to chromosome missegregation. This manuscript describes chromatin spread preparation methods for mouse prophase, metaphase I and II-staged oocytes. These fundamental techniques allow for the study of chromatin-bound proteins and chromosome morphology throughout mammalian oogenesis.

Tags

Keywords: Chromatin SpreadMouse OocyteProphaseMetaphase IIOogenesisChromosomeImmunolabelingPloidyAneuploidyInfertilityOvaryHypo-extraction BufferSucrose

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