JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
一种有效的
体外
转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
An Efficient
In Vitro
Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
Please note that all translations are automatically generated.
Click here for the English version.
一种有效的
体外
转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量
DOI:
10.3791/56742-v
•
10:13 min
•
January 12, 2018
•
Daniela Benati
,
Fabienne Cocchiarella
,
Alessandra Recchia
1
Centre for Regenerative Medicine, Department of Life Sciences
,
University of Modena and Reggio Emilia
Chapters
00:05
Title
00:51
Transduction of Human Primary Keratinocytes
04:31
Cytofluorimetric Analysis of Transduced Primary Keratinocytes
06:24
Quantitative RT-PCR (qRT-PCR)
07:24
Results: Sleeping Beauty System Achieves Stable Integration of a GOI in the Human Genome
08:40
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议描述了一种方法, 以实现稳定的基因的兴趣进入人类基因组由转录调控的
睡美人
系统。本文报道了有缺陷的慢载体的合成、人体细胞的
体外
转导和转细胞的分子检测。
Tags
Sleeping Beauty System
Integration Defective Lentiviral Vector
Gene Therapy
Primary Cells
Transcriptionally Regulated
Transposition Method
In Vitro
Hyperactive SB100X Transposase
Gene Of Interest
Tetracycline-regulated
Polybrene
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/56742?article_type=v
瞬时转染293T细胞复制缺陷型逆转录病毒生产
check_url/56742?article_type=v
自制的网站定向诱变整个质粒
check_url/56742?article_type=v
人类体细胞重编程为诱导多能干细胞(iPS细胞)用绿色荧光蛋白逆转录病毒载体
check_url/56742?article_type=v
一个循序渐进的步骤方法的到Nanodisc脂质颗粒的ABC转运蛋白的重组
check_url/56742?article_type=v
高通量筛选转录后调控基因的化学调节剂
check_url/56742?article_type=v
混合式切割:用于顽拗植物组织样品的改进切法
check_url/56742?article_type=v
一种用于研究卡波西肉瘤细胞转化的
体外
模型
check_url/56742?article_type=v
三维球体制作一个高效,灵活细胞聚集方法
check_url/56742?article_type=v
IR-TEx:专为疟疾病媒阿
诺菲斯甘巴病
设计的大数据转录组学的开源数据集成工具
check_url/56742?article_type=v
通过粘附足迹测定法对细胞滚动中的分子粘附进行成像
Read Article