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一种有效的体外转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量
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JoVE Journal Biology
An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
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一种有效的体外转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量

DOI:
10.3791/56742-v

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10:13 min

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January 12, 2018

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, ,
1Centre for Regenerative Medicine, Department of Life Sciences,University of Modena and Reggio Emilia

Chapters

  • 00:05Title
  • 00:51Transduction of Human Primary Keratinocytes
  • 04:31Cytofluorimetric Analysis of Transduced Primary Keratinocytes
  • 06:24Quantitative RT-PCR (qRT-PCR)
  • 07:24Results: Sleeping Beauty System Achieves Stable Integration of a GOI in the Human Genome
  • 08:40Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一种方法, 以实现稳定的基因的兴趣进入人类基因组由转录调控的睡美人系统。本文报道了有缺陷的慢载体的合成、人体细胞的体外转导和转细胞的分子检测。

Tags

Sleeping Beauty SystemIntegration Defective Lentiviral VectorGene TherapyPrimary CellsTranscriptionally RegulatedTransposition MethodIn VitroHyperactive SB100X TransposaseGene Of InterestTetracycline-regulatedPolybrene

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