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转录因子结合、转录、翻译和营业额的实时分析在细胞激活过程中显示全球事件

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Please note that all translations are automatically generated. Click here for the English version.

转录因子结合、转录、翻译和营业额的实时分析在细胞激活过程中显示全球事件

DOI:

10.3791/56752-v

•

12:54 min

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March 07, 2018

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Kathrin Davari*¹, Johannes Lichti*¹, Caroline C. Friedel, Elke Glasmacher³

¹Institute for Diabetes and Obesity (IDO), German Center for Diabetes Research (DZD),Helmholtz Zentrum München, ²Institute for Informatics,Ludwig-Maximilians-Universität München, ³Roche Pharma Research and Early Development, Large Molecule Research,Roche Innovation Center Penzberg

Chapters

00:05Title
00:46Profiling Recently Transcribed RNA
03:52Profiling RNA in the Process of Translation
08:01Profiling Transcription Factor Binding
10:35Results: Quality Control Tests
12:05Conclusion

Summary

Automatic Translation

该协议描述了芯片序列的组合使用, 4 的 su 序列, 总 RNA 序列, 和核糖体分析的细胞系和主细胞。它能够跟踪转录因子绑定、从头转录、RNA 处理、营业额和翻译的变化, 并在激活和/或快速变化的细胞中显示事件的总体过程。

Tags

Real-time Analysis Transcription Factor Binding Transcription Translation Turnover Cellular Activation RNA Regulation DNA Regulation Molecular Mechanisms Immune Response Drug Treatment 4sU Labeling Newly Transcribed RNA Biotinylation Streptavidin Beads

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