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毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

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毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测

DOI:

10.3791/56794-v

•

07:58 min

•

September 19, 2018

•

Narendra Padhan

¹Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory,751 85, Uppsala, Sweden

Chapters

00:04Title
01:00Sample Mix Preparation (Calculation for 150 L Sample Mix)
03:03Designing a New Assay Template with System Software
04:33Running the Assay File
05:20Analyze the Data
05:46Results: Characterization of Extracellular Signal Regulated Kinase Proteins by Capillary Isoelectric Focusing
07:08Conclusion

Summary

Automatic Translation

毛细管等电聚焦是一种基于抗体的、超灵敏的高通量技术, 它能够从极小的生物样品中详细描述蛋白质及其亚型。下面介绍了一种自动和机器人的方法, 用于检测和量化特定蛋白质及其亚型。

Tags

Capillary Isoelectric Focusing Protein Detection Protein Isoforms Biomarker Discovery Drug Discovery Diagnostics High-throughput Reproducible Cell-signaling Pathways Protein Analysis Sample Preparation Isoelectric Point Standard Ladder Antibody Dilution Luminol-peroxide Detection

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