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Biochemistry
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毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测
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Biochemistry
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JoVE Journal
Biochemistry
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Please note that all translations are automatically generated.
Click here for the English version.
毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测
DOI:
10.3791/56794-v
•
07:58 min
•
September 19, 2018
•
Narendra Padhan
1
Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory
,
751 85, Uppsala, Sweden
Chapters
00:04
Title
01:00
Sample Mix Preparation (Calculation for 150 L Sample Mix)
03:03
Designing a New Assay Template with System Software
04:33
Running the Assay File
05:20
Analyze the Data
05:46
Results: Characterization of Extracellular Signal Regulated Kinase Proteins by Capillary Isoelectric Focusing
07:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
毛细管等电聚焦是一种基于抗体的、超灵敏的高通量技术, 它能够从极小的生物样品中详细描述蛋白质及其亚型。下面介绍了一种自动和机器人的方法, 用于检测和量化特定蛋白质及其亚型。
Tags
Capillary Isoelectric Focusing
Protein Detection
Protein Isoforms
Biomarker Discovery
Drug Discovery
Diagnostics
High-throughput
Reproducible
Cell-signaling Pathways
Protein Analysis
Sample Preparation
Isoelectric Point Standard Ladder
Antibody Dilution
Luminol-peroxide Detection
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