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Bruk fluorescens In Situ hybridisering (fisk) å overvåke tilstanden til Arm samhold i Prometaphase og Metaphase jeg Drosophila Oocytes
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes
DOI:

12:46 min

December 06, 2017

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Chapters

  • 00:05Title
  • 00:50Removal of Chorions and Vitelline Membranes
  • 05:57FISH
  • 10:27Results: Representative Images of Meiotic Figures with Intact Arm Cohesion and Disrupted Arm Cohesion
  • 11:31Conclusion

Summary

Automatic Translation

Dette manuskriptet presenterer en detaljert metode for å generere X-kromosom arm sonder og utføre fluorescens i situ hybridisering (fisk) å undersøke tilstanden til søster chromatid samhold i prometaphase og metaphase jeg arrestert Drosophila oocytes. Denne protokollen er egnet for å bestemme om meiotic arm samhold er intakt eller forstyrret i ulike genotyper.

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