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核糖体剖面对芽殖酵母中翻译的全基因组定量
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Biochemistry
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JoVE Journal Biochemistry
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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核糖体剖面对芽殖酵母中翻译的全基因组定量

DOI:
10.3791/56820-v

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12:57 min

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December 21, 2017

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, , ,
1Department of Dermatology,Boston University School of Medicine

Chapters

  • 00:05Title
  • 00:52Extract Preparation
  • 02:43Footprint Extraction
  • 05:15Dephosphorylation
  • 07:07Reverse Transcription
  • 10:17Results: Ribo-seq Enables Genome-wide Quantification of Translation of Specific mRNAs Under Different Cellular Conditions
  • 11:59Conclusion

Summary

Automatic Translation

Automatic Translation

翻译调控在蛋白质丰度控制中起着重要的作用。在这里, 我们描述一个高通量的方法, 定量分析翻译在萌芽酵母酿酒酵母。

Tags

Ribosome ProfilingGenome-wideTranslationBudding YeastSaccharomyces CerevisiaeProtein TranslationTranslational RegulationCell LysatePoly(A) MRNASucrose GradientRNase ICycloheximide

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