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Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

DOI:
10.3791/56888-v

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10:15 min

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December 09, 2017

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1Department of Pediatrics/Child Neurology, Amsterdam Neuroscience,VU University Medical Center, 2Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

Chapters

  • 00:05Title
  • 00:443D “Organoid” Differentiation
  • 04:32Alternative 2D Differentiation Culture
  • 08:28Results: 3D Products Exhibit Markers and Structures of Early Neuroepithelium, and 2D Products Exhibit Cerebellar Markers and Functional Neuronal Activity
  • 09:48Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a simplified 3D differentiation protocol for hPSCs, using defined medium and reduced growth factors, capable of generating cell aggregates with early neuroepithelial structures and positive for cerebellar-associated markers, as well as an optional 2D modification for differentiating cells as a monolayer to generate functional neurons.

Tags

Cerebellar DifferentiationNeural Differentiation3D Organoids2D MonolayerHuman Pluripotent Stem CellsFGF2ROCK InhibitorStreamlined ProtocolCost-effectiveDisease Modeling

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