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Behavior

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JoVE Journal Behavior
Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila
 

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Article DOI: 10.3791/56900-v • 07:24 min • April 24th, 2018
April 24th, 2018 •
*1,2, *2,3, 2,3
1School of Life Science, University of Science and Technology of China, 2State key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences
* These authors contributed equally

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Summary
Automatic Translation

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

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Keywords: Food Intake Drosophila Quantitative Assay Thermogenetic Activation Optogenetic Activation Hunger Satiety Appetite Agarose Pad Sucrose Blue Dye Feeding Chamber Temperature
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