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Journal
/
Neuroscience
/
比率钙显像对单个神经元的行为
线虫线虫
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Ratiometric Calcium Imaging of Individual Neurons in Behaving
Caenorhabditis Elegans
Please note that all translations are automatically generated.
Click here for the English version.
比率钙显像对单个神经元的行为
线虫线虫
DOI:
10.3791/56911-v
•
11:26 min
•
February 07, 2018
•
Bhavya Ravi*
1
,
Layla M. Nassar*
1,2
,
Richard J. Kopchock III*
2
,
Pravat Dhakal*
2
,
Michael Scheetz
,
Kevin M. Collins
2
1
Neuroscience Program
,
University of Miami School of Medicine
,
2
Department of Biology
,
University of Miami
Chapters
00:04
Title
01:03
Animal Mounting
02:44
Imaging Setup
05:36
Ratiometric HSN Ca
2
Imaging and
C. elegans
Behavior Recording
07:09
Image Segmentation and Quantitative Analysis
08:58
Results: Representative HSN Imaging During Egg-laying
10:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了使用基因编码的 Ca
2
记者记录在行为上的神经活动的变化, 在
线虫线虫
蠕虫。
Tags
Keywords: Ratiometric Calcium Imaging
Caenorhabditis Elegans
HSN Neuron
Behavioral Neuroscience
GCaMP5
MCherry
Nematode Growth Medium
OP50 Bacteria
Vulva
Inverted Microscope
Infrared Filter
Video Analysis
Stage XY Stage Control
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