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Genome-wide RNAi Screening per identificare i fattori ospite che modulano la terapia Virus Oncolytic
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1Children’s Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology,University of Ottawa, 3Department of Pediatrics,University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine,University of Calgary
Chapters
- 00:05Title
- 00:45Preparation for Reverse Transcription
- 01:57Reverse Transfection
- 04:32Infection
- 05:52Fixing and Staining Cells
- 06:39Results: Genome-wide RNAi Screen to Identify Host Targets that Enhance Oncolytic Virus Therapy
- 08:13Conclusion
Qui descriviamo un protocollo per impiegando HTS RNAi per scoprire destinazioni di host che possono essere manipolate per migliorare la terapia virus oncolytic, specificamente terapia del rhabodvirus e del vaccinia virus, ma può essere facilmente adattata ad altri oncolytic piattaforme di virus o di scoperta di geni ospitanti che modulano la replicazione del virus in generale.
