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Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
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Chapters
- 00:05Title
- 00:38Assessment of Binding Between Notch Receptor and Trans-ligands
- 01:47Aggregation Between Signal-sending and Signal-receiving Cells
- 03:11Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands
- 04:15Results: Faithful Analysis of Notch Signaling
- 05:30Conclusion
Complexity of in vivo systems makes it difficult to distinguish between the activation and inhibition of Notch receptor by trans- and cis-ligands, respectively. Here, we present a protocol based on in vitro cell-aggregation assays for qualitative and semi-quantitative evaluation of the binding of Drosophila Notch to trans-ligands vs cis-ligands.
