/

Cancer Research

/

用医用金属丝捕获循环肿瘤细胞的肿瘤细胞: 基于免疫荧光和 DNA 的3D 方法

JoVE Journal
Cancer Research

This content is Free Access.

JoVE Journal Cancer Research

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH

Please note that all translations are automatically generated. Click here for the English version.

用医用金属丝捕获循环肿瘤细胞的肿瘤细胞: 基于免疫荧光和 DNA 的3D 方法

DOI:

10.3791/56936-v

•

10:56 min

•

December 21, 2017

•

Giulia Gallerani, Claudia Cocchi, Martine Bocchini, Filippo Piccinini, Francesco Fabbri

¹Biosciences Laboratory,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, ²Associazione Annastaccatolisa Onlus, ³Nuclear Medicine Unit,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Chapters

00:05Title
01:34Immuno-DNA FISH (Fluorescent In Situ Hybridization) on Wire
02:35Fixation, Permeabilization, and Immunofluorescence
04:253D DNA FISH Day 2
06:083D DNA FISH Day 3
07:513D Microscope Observation and Analysis
09:01Results: No Interference Bewteen Immunofluorescence and DNA FISH Signals
09:38Conclusion

Summary

Automatic Translation

我们提出了一种新的方法来描述肿瘤细胞。我们结合免疫荧光与 DNA 荧光-原位杂交, 以评估细胞捕获的功能性医用电线, 能够在体内的,丰富 ctc 直接从病人的血液。

Tags

Keywords: Tumor Cells Circulating Tumor Cells 3D Approach Immunofluorescence DNA FISH EpCAM Cytosomatic Alterations ARC Gene Non-small-cell Lung Cancer Breast Cancer CTC Characterization In Situ Detection Immunophenotyping Fluorescence Microscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

捕获和血液中的活循环肿瘤细胞释放

该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克

在直肠癌原位小鼠模型中循环肿瘤细胞的分离

肺定植法在肺部组织循环肿瘤细胞可视化中的建立

数字聚合酶链反应法测定零星家族性腺瘤性息肉病患者的基因变异

循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估

患者生物流体中肿瘤相关循环DNA的检测与监测

免疫荧光对非小细胞肺癌患者循环肿瘤细胞的半自动PD-L1特征化及枚举

利用人类诱导多能干细胞生成肿瘤抗原特异性T细胞

通过高分辨率共聚焦显微镜对3D肿瘤球体入侵的影响表征

Read Article