/

Immunology and Infection

/

以层流为基础的检测培养血管细胞和粘附血小板白细胞的研究

JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Immunology and Infection

Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets

Please note that all translations are automatically generated. Click here for the English version.

以层流为基础的检测培养血管细胞和粘附血小板白细胞的研究

DOI:

10.3791/57009-v

•

08:50 min

•

April 09, 2018

•

Tanja Vajen, Alexandra C.A. Heinzmann, Annemiek Dickhout, Zhen Zhao, Magdolna Nagy, Johan W.M. Heemskerk, Rory R. Koenen

¹Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, ²Department of Vascular Surgery, Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University, School of Medicine

Chapters

00:05Title
00:57Flow-based Assay with Human Cells
02:28Preparation of Adherent Platelet and Vascular Cell Monolayers
03:56Fluorescent Labeling and Perfusion of Leukocytes
06:32Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
08:09Conclusion

Summary

Automatic Translation

在血管壁损伤或炎症期间, 白细胞与血管细胞和血小板相互作用。在这里, 我们描述了一种简单的基于层流的检测方法来表征白细胞与其细胞伙伴之间相互作用的分子机制。

Tags

Keyword Extraction:Leukocyte Recruitment Adherent Vascular Cells Adherent Platelets Flow Conditions Laminar Flow-based Assays Immunology Leukocyte Adhesion Tumor Cell Adhesion Platelet-rich Plasma Platelet-poor Plasma Platelet Buffer Hydrochloric Acid Ethanol Rat Collagen Type One Phosphate Buffered Saline

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

成像白细胞粘附到血管内皮细胞在高腔内压力

昆虫<em>大蜡螟</em作为一个强大的的感染模型探讨细菌致病

一个流程粘附实验来研究白细胞招聘剪切应力的人力肝窦内皮细胞条件下

猪或牛光感受器外段的细胞吞噬分析对视网膜色素上皮细胞大规模纯化

对白细胞从流程的分析招聘基质细胞的影响

在体外和体内模型来研究细菌粘附于血管壁下流动条件

在Mesenterial静脉的活体显微镜白细胞，内皮细胞和血小板白细胞相互作用小鼠

辅助性T细胞作为一种遗传方法来研究逆转录病毒转导机制控制其分化和功能

荧光为基础的神经氨酸酶抑制测定流感病毒的易感性评估抗病毒药的神经氨酸酶抑制剂类

应用系统进化分析研究真核基因的起源

Read Article