Journal
/
/
Laminar Flow-baseret Assays til at undersøge leukocyt ansættelse på kulturperler vaskulære celler og vedhængende blodplader
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
DOI:

08:50 min

April 09, 2018

, , , , , ,

Chapters

  • 00:05Title
  • 00:57Flow-based Assay with Human Cells
  • 02:28Preparation of Adherent Platelet and Vascular Cell Monolayers
  • 03:56Fluorescent Labeling and Perfusion of Leukocytes
  • 06:32Results: Use of Laminar Flow-based Assay to Investigate the Interactions of Leukocytes with Their Cellular Partners
  • 08:09Conclusion

Summary

Automatic Translation

Leukocytter interagere begærligt med vaskulære celler og blodplader efter fartøjet væg skade eller under betændelse. Her, beskriver vi en ligetil laminar flow-baseret analyse for at karakterisere de molekylære mekanismer, der ligger til grund for interaktioner mellem leukocytter og deres cellulære partnere.

Related Videos

Read Article