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Native kromatin Immunoprecipitation ved hjælp af Murine hjerne Tumor Neurospheres
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1Department of Cell and Developmental Biology,University of Michigan Medical School, 2Department of Neurosurgery,University of Michigan Medical School, 3Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program,University of Michigan Medical School, 4Department of Biology,University of Puerto Rico-Río Piedras Campus
Chapters
- 00:05Title
- 00:40Generation of Brain Tumor Neurospheres and Culturing Conditions
- 02:23Chromatin Preparation
- 04:52Immunoprecipitation (IP)
- 08:21Results: Representative ChIP qPCR Data as Percent Input
- 09:05Conclusion
Epigenetiske mekanismer er ofte ændret i gliom. Kromatin immunoprecipitation kunne bruges til at studere konsekvenserne af genetiske ændringer i gliom, der skyldes ændringer i Histon ændringer, som regulerer kromatin struktur og gen transskription. Denne protokol beskriver indfødte kromatin immunoprecipitation på murine hjerne tumor neurospheres.
