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Mouse Adipose Tissue Collection and Processing for RNA Analysis
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Biochemistry
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JoVE Journal Biochemistry
Mouse Adipose Tissue Collection and Processing for RNA Analysis

Mouse Adipose Tissue Collection and Processing for RNA Analysis

DOI:
10.3791/57026-v

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11:13 min

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January 31, 2018

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, ,
1Centre de Recherche du Centre Hospitalier,Université de Montréal, 2Department of Biochemistry and Molecular Medicine,Université de Montréal, 3Department of Kinesiology,Université de Montréal, 4Montreal Diabetes Research Center

Chapters

  • 00:05Title
  • 00:52Necropsy and Adipose Tissue Collection from Male Mice
  • 03:38Preparation of Ground White Adipose Tissue for RNA Isolation
  • 04:59RNA Isolation from Ground Adipose Tissue
  • 09:07Results: RNA Analysis of Mouse Adipose Tissue
  • 10:12Conclusion

Summary

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The purpose of this paper is to present a step-by-step procedure to collect different white adipose tissues from mice, process the fat samples and extract RNA.

Tags

RNA AnalysisMetabolismBiochemistryMolecular BiologyGene ExpressionSubcutaneous FatPerigonadal FatPerirenal FatLiquid Nitrogen

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