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Een Time-lapse, Label-vrije, kwantitatieve fase Imaging studie van slapende en actieve menselijke kankercellen
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
DOI:

12:48 min

February 16, 2018

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Chapters

  • 00:04Title
  • 00:58Cell Preparation
  • 02:35Cell Preparation: Splitting KHOS-A and KHOS-N Cells
  • 03:50Cell Preparation: Seeding KHOS-A and KHOS-N Cells for QPI
  • 04:30Quantitative Phase Image Acquisition: Cover Slip Set-up
  • 05:19Quantitative Phase Imaging
  • 07:03Analyzing Cell Morphology
  • 08:20Analyzing Cell Proliferation
  • 08:54Analyzing Cell Motion
  • 10:16Results: Quantiative Phase Imaging of Dormant and Active Human Cancer Cells
  • 11:50Conclusion

Summary

Automatic Translation

Slapende en actieve kanker cel fenotypen werden gekenmerkt met behulp van kwantitatieve fase imaging. Cel proliferatie, migratie en morfologie assays werden geïntegreerd en geanalyseerd in één eenvoudige methode.

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