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Journal
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Immunology and Infection
/
全场光学相干显微镜,用于角膜移植物基质特征的组织学样分析
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Please note that all translations are automatically generated.
Click here for the English version.
全场光学相干显微镜,用于角膜移植物基质特征的组织学样分析
DOI:
10.3791/57104-v
•
07:51 min
•
October 21, 2022
•
Kristina Irsch
2,3
,
Kate Grieve
2
,
Marie Borderie
,
Maëlle Vilbert
2,4
,
Karsten Plamann
5
,
Djida Ghoubay
2
,
Cristina Georgeon
,
Vincent Borderie
2
1
Vision Institute
,
Sorbonne University, UM 80 / INSERM, UMR_S 968 / CNRS, UMR_7210
,
2
Quinze Vingts National Ophthalmology Hospital
,
3
Laboratory of Ophthalmic Instrument Development, The Wilmer Eye Institute
,
Johns Hopkins University School of Medicine
,
4
Laboratory for Optics and Biosciences (LOB)
,
École polytechnique
,
5
LOA – ENSTA Paris
,
École polytechnique
Chapters
00:04
Introduction
00:47
Sample Preparation
01:31
FF‐OCM Initialization, Setup, and Image Acquisition
03:48
Image Analysis: Qualitative and Quantitative Assessment of Stromal Morphology and Features
06:08
Results: Qualitative and Quantitative Assessment of Corneal Donor Stroma
07:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们描述了使用全场光学相干显微镜作为高质量评估角膜供体基质的方法。该协议可用于识别指示健康或疾病的特征,旨在改善供体组织的筛选和选择,从而改善角膜移植术的结果。
Tags
Cornea
Corneal Graft
Optical Coherence Microscopy
Histology
Stromal Features
Eye Banks
Cornea Transplantation
High Resolution
Immersion Medium
Sample Holder
Cover Slip
Green LED
Acquisition Software
Macro Image
Auto-adjustment
Joystick
Depth Adjustment
Averaging
Corneal Surface
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