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用于研究植物-微生物相互作用的实验室生态系统制造 (EcoFAB) 协议

JoVE Journal
Environment

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JoVE Journal Environment

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

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用于研究植物-微生物相互作用的实验室生态系统制造 (EcoFAB) 协议

DOI:

10.3791/57170-v

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11:57 min

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April 10, 2018

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Jian Gao², Joelle Sasse², Kyle M. Lewald², Kateryna Zhalnina², Lloyd T. Cornmesser², Todd A. Duncombe, Yasuo Yoshikuni, John P. Vogel, Mary K. Firestone, Trent R. Northen²

¹Environmental Genomics and Systems Biology Division,Lawrence Berkeley National Laboratory, ²Joint Genome Institute,Department of Energy, ³Joint BioEnergy Institute, ⁴Department of Environmental Science Policy and Management,University of California

Chapters

00:04Title
01:13EcoFAB Device Fabrication: Casting PDMS Layers
03:22EcoFAB Device Fabrication: Chemically Attached PDMS Layers onto Microscope Slides
04:50EcoFAB Sterilization
05:59Growing Plants in EcoFABs
08:31Results: Plant-microbe Interactions Using Ecosystem Fabrication Protocols
10:18Conclusion

Summary

Automatic Translation

本文描述了设备 (EcoFABs) 的生态系统制造的详细协议, 它能够在高控制的实验室条件下对植物和植物微生物相互作用进行研究。

Tags

Ecosystem Fabrication (EcoFAB)Plant-microbe Interactions Laboratory Ecosystems 3D Printing PDMS Microscope Slides Root Morphology Mass Spectroscopic Imaging Sustainable Agriculture

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