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Chapters
- 00:04Title
- 01:05Handling of the Peptide and Cells
- 03:02Binding of His-tagged Proteins to the HisPur Cobalt Beads
- 04:39Binding of the Peptide to the His-tagged Protein Immobilized on the Beads
- 05:59Elution from Cobalt Beads
- 07:07Electrophoresis, Western Blotting, and Analysis of Results
- 07:58Results: Use of Immunoblot Assay to Study the Interaction between Sortilin Immobilized on Cobalt Beads and myc-tagged Peptide
- 09:18Conclusion
