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Immunology and Infection

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腹膜源性肥大细胞的分离及其功能特征的 Ca^{2 +}成像和脱粒测定

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Immunology and Infection

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JoVE Journal Immunology and Infection

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca²⁺-imaging and Degranulation Assays

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腹膜源性肥大细胞的分离及其功能特征的 Ca^{2 +}成像和脱粒测定

DOI:

10.3791/57222-v

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11:31 min

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July 04, 2018

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Volodymyr Tsvilovskyy, Alejandra Solis-Lopez, Kathrin Öhlenschläger, Marc Freichel

¹Institute of Pharmacology,Ruprecht-Karls Heidelberg University

Chapters

00:04Title
00:38PMC Isolation and Cultivation by Intraperitoneal Lavage
03:14Fluorometric Intracellular Free Calcium Concentration Measurement in PMCs
06:38Beta-hexosaminidase Release Measurement in PMCs
08:46Results: Beta-hexosaminidase Release Colorimetric Multi-well Measurements Performed with PMCs Isolated from Wild Type Mice
10:14Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个隔离和培养小鼠腹膜肥大细胞的协议。我们还描述了两个协议的功能表征: 一个荧光成像的细胞内游离 Ca^{2 +}浓度和脱粒检测的基础上的色度量化的释放β-hexosaminidase。

Tags

Keywords: Peritoneum Mast Cells Calcium Imaging Degranulation Intracellular Signaling Excitable Cells Ion Channels Immune Cell Activation Mediator Release Fura-2 RPMI Medium Peritoneal Cavity Cell Suspension IL-3 SCF IgE Receptors

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