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Electrochemiluminescence Assays for Human Islet Autoantibodies
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Electrochemiluminescence Assays for Human Islet Autoantibodies

Electrochemiluminescence Assays for Human Islet Autoantibodies

DOI:
10.3791/57227-v

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09:15 min

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March 23, 2018

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, , , , ,
1Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, 2Department of Endocrinology,First Affiliated Hospital of Nanjing Medical University

Chapters

  • 00:05Title
  • 01:51Labeling of the Human Islet Autoantigen Followed by Incubation of the Serum Samples with the Labeled Antigens
  • 05:01Preparation of the Streptavidin Plate, Transfer of the Serum/Antigen Incubate, and Reading of the Streptavidin Plate
  • 06:32Results: Data Analysis
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we presented the methods to detect human islet autoantibodies using electrochemiluminescence (ECL) assays. The protocol, used to predict type 1 diabetes, can be expanded upon to detect autoantibodies for other autoimmune diseases.

Tags

ElectrochemiluminescenceIslet AutoantibodiesType 1 DiabetesAutoimmuneNon-radioactiveRadioassayELISAInsulin AutoantibodyBiotinSULFO-TAGSpin ColumnCheckerboard AssayPhosphate Buffered Saline

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