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利用常规 PCR 技术对柑桔叶片组织样品Candidatus liberibacter的特异和准确检测

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Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Please note that all translations are automatically generated. Click here for the English version.

利用常规 PCR 技术对柑桔叶片组织样品Candidatus liberibacter的特异和准确检测

DOI:

10.3791/57240-v

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09:23 min

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June 29, 2018

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Huan Chen*^1,2, Ian Arthur Palmer*¹, Jian Chen², Ming Chang², Stephen L. Thompson, Fengquan Liu, Zheng Qing Fu

¹Department of Biological Sciences,University of South Carolina, ²Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences, ³Department of Instructions and Teacher Education,University of South Carolina

Chapters

00:04Title
00:49Isolation of Genomic DNA from Plant Tissue
05:04Perform PCR on Genomic DNA Samplesand Electrophoresis of the Amplified DNA II. Isolation of Genomic DNA from Plant Tissueon an Agarose Gel
07:34Results: PCR Amplification of Genomic DNA to Identify the Citrus Greening Bacterium
08:52Conclusion

Summary

Automatic Translation

柑橘类绿化是影响全球柑橘作物的一种特殊破坏性疾病。本文介绍了一种利用 PCR 和基因组 DNA 提取柑桔叶组织的简便方法, 对柑桔绿化病原体、 Candidatus liberibacter 进行准确、准确的鉴定。

Tags

Keywords: Citrus Greening Candidatus Liberibacter Spp.PCR Detection Pathogen Identification Citrus Farming Quantitative Analysis Laboratory Procedure RNA Removal DNA Precipitation Centrifugation

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