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微流控装置中组织模型的 Perfusable 血管网
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Bioengineering
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JoVE Journal Bioengineering
Perfusable Vascular Network with a Tissue Model in a Microfluidic Device
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微流控装置中组织模型的 Perfusable 血管网

DOI:
10.3791/57242-v

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07:05 min

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April 04, 2018

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, , , , , , , , ,
1Department of Micro Engineering,Kyoto University, 2Graduate School of Medical Sciences,Kyushu University, 3International Research Center for Medical Sciences (IRCMS),Kumamoto University

Chapters

  • 00:04Title
  • 00:48Polydimethylsiloxane (PDMS) Layer Fabrication and Assembly
  • 01:48Microfluidic Device Cell Seeding
  • 05:12Results: Representative Vascular Lumen Formation
  • 06:42Conclusion

Summary

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该协议描述了如何在球体中设计一个 perfusable 的血管网。球体周围的微环境被设计来诱导血管生成, 并将球体连接到微流控装置中的微。该方法允许球状的灌注, 这是一个期待已久的技术在三维文化。

Tags

Keywords: Microfluidic DevicePerfusable Vascular NetworkSpheroidHLFRFP-HUVECGFP-HUVECEndothelial MediumMaster Mix SolutionRegenerative Medicine

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