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Journal
/
Developmental Biology
/
催不整脈性心筋症患者の心内膜心筋生検サンプルから心臓の間葉系間質細胞の分離と性状
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Isolation and Characterization of Cardiac Mesenchymal Stromal Cells from Endomyocardial Bioptic Samples of Arrhythmogenic Cardiomyopathy Patients
Please note that all translations are automatically generated.
Click here for the English version.
催不整脈性心筋症患者の心内膜心筋生検サンプルから心臓の間葉系間質細胞の分離と性状
DOI:
10.3791/57263-v
•
09:16 min
•
February 28, 2018
•
Chiara Assunta Pilato*
1,2
,
Ilaria Stadiotti*
1
,
Angela Serena Maione
,
Valentina Saverio
,
Valentina Catto
,
Fabrizio Tundo
,
Antonio Dello Russo
,
Claudio Tondo
3
,
Giulio Pompilio
2
,
Michela Casella*
3
,
Elena Sommariva*
1
1
Vascular Biology and Regenerative Medicine Unit
,
Centro Cardiologico Monzino-IRCCS
,
2
Department of Clinical Sciences and Community Health
,
Università degli Studi di Milano
,
3
Heart Rhythm Center
,
Centro Cardiologico Monzino-IRCCS
Chapters
00:04
Title
01:12
Cardiac Biopsy Collection and Processing
03:06
Characterizing Cardiac Mesenchymal Stromal Cells (C-MSC) by Flow Cytometry
05:22
Oil Red O (ORO) Staining C-MSC after Adipogenic Differentiation
07:02
Results: Flow Cytometry Analysis and ORO Staining
08:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
この記事では、催不整脈性心筋症患者の心内膜心筋生検サンプルから心臓の間葉系間質細胞を分離するメソッドが提供されます。その特性とその脂肪細胞分化を促進するためのプロトコルを説明します。
Tags
Keywords: Cardiac Mesenchymal Stromal Cells
Arrhythmogenic Cardiomyopathy
Endomyocardial Biopsy
Adipogenic Differentiation
Phenotyping
Cell Isolation
Cell Culture
Collagenase Digestion
Surface Markers
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