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Humane pluripotente stamceller kultur på Polyvinyl alkohol-Co-Itaconic syre Hydrogels med varierende stivhed på Xeno-fri betingelser
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1Department of Chemical and Materials Engineering,National Central University, 2Department of Botany and Microbiology,King Saud University, 3Cathay Medical Research Institute,Cathay General Hospital, 4Graduate Institute of Systems Biology and Bioinformatics,National Central University, 5Department of Medical Microbiology and Parasitology,Universiti Putra Malaysia, 6Department of Internal Medicine,Taiwan Landseed Hospital, 7Department of Zoology,Bharathiar University, 8Thiruvalluvar University
Chapters
- 00:04Title
- 01:00Polyvinyl Alcohol-Co-Itaconic Acid (P-IA) Hydrogel Dish Preparation and Grafting
- 05:02Passaging of Undifferentiated Human Embryonic Stem (ES) and Human Induced Pluripotent Stem (iPS) Cells on Hydrogels
- 06:37Characterization of the Expression of Pluripotency Proteins by Immunostaining
- 08:49Results: Human ES and iPS Pluripotency on Conventional Dishes and Hydrogel Dishes
- 10:19Conclusion
En protokol, der er forelagt for at forberede polyvinyl alkohol-co-itaconic syre hydrogels med varierende stivhed, som blev podet med og uden oligopeptides, for at undersøge effekten af stivhed af biomaterialer på differentiering og spredning af stamceller. Stivhed af hydrogels blev kontrolleret af crosslinking tid.
