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JoVE Journal Bioengineering
Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
 

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Article DOI: 10.3791/61299-v • 08:06 min • July 2nd, 2020
July 2nd, 2020 •
1, 1, 1, 1,2
1Department of Biomedical Engineering, Duke University, 2Department of Medicine, Division of Nephrology, Duke University School of Medicine

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Summary
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Presented here is a chemically defined protocol for the derivation of human kidney podocytes from induced pluripotent stem cells with high efficiency (>90%) and independent of genetic manipulations or subpopulation selection. This protocol produces the desired cell type within 26 days and could be useful for nephrotoxicity testing and disease modeling.

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Keywords: Human Induced Pluripotent Stem Cells Kidney Podocytes Chemically Defined Conditions Feeder-free Culture Cell Differentiation Basement Membrane Matrix Dissociation Cell Culture Kidney Disease Modeling
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