JoVE Journal
Biochemistry
Biochemistry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
A detailed protocol to perform a titration ELISA is described. Moreover, a novel algorithm is presented to evaluate titration ELISAs and to obtain a dissociation constant of binding of a soluble ligand to a microtiter plate-immobilized receptor.