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1Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Copenhagen, 2Department of Anesthesiology,Yale School of Medicine, 3Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Rochester Medical Center, 4Department of Experimental Medical Science, Wallenberg Center for Molecular Medicine,Lund University
Chapters
- 00:04Title
- 01:17Cannula Preparation
- 01:53Insertion of the Cannula into the Cisterna Magna for Chronic Injections of CSF Tracers
- 05:52Injection of CSF Tracers
- 06:43Injecting CSF Tracers in Anesthetized Mice with Chronic Cisterna Magna Cannulation
- 07:20Injecting CSF Tracers in Awake Mice with Chronic Cisterna Magna Cannulation
- 08:05Results: Analysis of CSF Tracer Distribution
- 09:02Conclusion
Here we describe a protocol to perform cisterna magna cannulation (CMc), a minimally invasive way to deliver tracers, substrates and signaling molecules into the cerebrospinal fluid (CSF). Combined with different imaging modalities, CMc enables glymphatic system and CSF dynamics assessment, as well as brain-wide delivery of various compounds.
