Journal
/
/
rna 下拉法识别长非编码 rna 的 rna 靶点
JoVE Journal
Genetics
This content is Free Access.
JoVE Journal Genetics
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
DOI:

09:36 min

April 10, 2018

, , , , , , ,

Chapters

  • 00:04Title
  • 01:29Cultured Cell and Tissue Crosslinking, Lysis, and Sonication
  • 04:09RNA Pull-down
  • 06:33Results: RNA Pull-down to Identify Targets of IncRNAs, Neat1, and Malat1
  • 08:25Conclusion

Summary

Automatic Translation

这种 rna 下拉法允许识别长的非编码 rna (lncRNA) 的 rna 目标。根据自制的、设计的抗感 DNA 寡核苷酸探针的杂交, 在适当的固定组织或细胞系 lncRNA, 它有效地允许捕获 lncRNA 的所有 RNA 目标。

Related Videos

Read Article