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Live Cell Imaging of Chromosome Segregation During Mitosis
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Developmental Biology
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JoVE Journal Developmental Biology
Live Cell Imaging of Chromosome Segregation During Mitosis

Live Cell Imaging of Chromosome Segregation During Mitosis

DOI:
10.3791/57389-v

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06:39 min

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March 14, 2018

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, ,
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

Chapters

  • 00:04Title
  • 01:04Labeling Cells
  • 02:21Visualization of Lagging Chromosomes
  • 04:45Results: Nocodazole-treated MEFs Lacking PP2A-B56 Undergo Chromosomal Mis-segregation
  • 05:29Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes an easy and convenient method to label and visualize live chromosomes in mitotic cells using Histone2B-GFP BacMam 2.0 label and a spinning disc confocal microscopy system.

Tags

Keywords: Live Cell ImagingChromosome SegregationMitosisHistone 2B-GFP BacMam 2.0Spinning Disc Confocal MicroscopyMouse Embryonic FibroblastsNocodazoleCell SynchronizationLive Chromosome VisualizationEnvironmental ChamberOil Immersion Objective

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