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评估溶酶体碱化在活的秀丽线虫中的肠道
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Developmental Biology
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JoVE Journal Developmental Biology
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
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评估溶酶体碱化在活的秀丽线虫中的肠道

DOI:
10.3791/57414-v

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06:18 min

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April 13, 2018

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,
1Department of Biology,University of Saskatchewan

Chapters

  • 00:04Title
  • 01:01Staining and Imaging of Intestinal Lysosomes
  • 04:24Results: Study of the Lysosomal Functional Activity Using a pH Sensitive Dye
  • 05:38Conclusion

Summary

Automatic Translation

Automatic Translation

用 pH 敏感的生命染料 5 (6)-羧基-2 ', 7 '-dichlorofluorescein 双乙酸 (cDCFDA) 研究了在线虫的肠道中溶酶体酸度损失的分步指南

Tags

Lysosomal AlkalinizationCaenorhabditis ElegansAutophagyLysosomal FunctionProtein DegradationCDCFDA StainingOP50 BacteriaNGM PlatesM9 BufferAgarose PadSodium Azide

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