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铜绿假单胞菌感染肺内皮细胞后细胞毒性 Amyloids 的检测方法
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Immunology and Infection
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JoVE Journal Immunology and Infection
Methods for Detecting Cytotoxic Amyloids Following Infection of Pulmonary Endothelial Cells by Pseudomonas aeruginosa
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铜绿假单胞菌感染肺内皮细胞后细胞毒性 Amyloids 的检测方法

DOI:
10.3791/57447-v

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07:27 min

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July 12, 2018

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, , ,
1Department of Biochemistry and Molecular Biology,University of South Alabama, 2Department of Physiology and Cell Biology,University of South Alabama, 3Department of Chemical and Biomolecular Engineering,University of South Alabama, 4Center for Lung Biology,University of South Alabama

Chapters

  • 00:04Title
  • 00:40Cytotoxic Supernatant Generation
  • 02:24Supernatant Cytotoxicity Analysis
  • 03:58Supernatant Amyloid Quantification
  • 05:27Results: Representative Supernatant Cytotoxicity Analyses
  • 06:58Conclusion

Summary

Automatic Translation

Automatic Translation

介绍了用铜绿假单胞菌感染肺内皮后细胞毒性 amyloids 的制备方法。

Tags

Keywords: Cytotoxic AmyloidsPseudomonas AeruginosaPulmonary Endothelial CellsCytotoxicitySupernatantCell MonolayerCell CultureMicroscopyImageJPulmonary CareHospital-acquired Pneumonia

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