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Immunology and Infection

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整合影像流式细胞仪和转录组分析, 评估改变的内吞 CD1d 贩运

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Immunology and Infection

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JoVE Journal Immunology and Infection

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

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整合影像流式细胞仪和转录组分析, 评估改变的内吞 CD1d 贩运

DOI:

10.3791/57528-v

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09:01 min

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October 29, 2018

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Manju Sharma, Xiang Zhang, Shouxiong Huang

¹Department of Environmental Health,University of Cincinnati College of Medicine

Chapters

00:04Title
01:14Pollutant-exposed Human Monocyte-derived Dendritic Cell (DC) Sequencing
04:10Imaging Flow Cytometry Analysis
05:42Results: CD1d and Lamp1 Co-localization Analysis
07:19Conclusion

Summary

Automatic Translation

成像流式细胞术提供了一种理想的方法来检测细胞在个体和种群水平的形态学和功能改变。对污染物暴露的人树突状细胞中脂质抗原表达的破坏内吞功能进行了转录组基因表达和蛋白质贩运形态表现的综合分析。

Tags

Keywords: Imaging Flow Cytometry Transcriptomic Profiling CD1d Trafficking Next-generation Sequencing Protein Function Dendritic Cells Sequencing Workflow

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