Journal
/
Medicine
/
Evaluering af Exon integration induceret af splejse skifte Antisense oligonukleotider i SMA patienten fibroblaster
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Evaluation of Exon Inclusion Induced by Splice Switching Antisense Oligonucleotides in SMA Patient Fibroblasts

Evaluering af Exon integration induceret af splejse skifte Antisense oligonukleotider i SMA patienten fibroblaster

DOI:
10.3791/57530-v

•

07:02 min

•

May 11, 2018

•

, ,
1Department of Medical Genetics,University of Alberta Faculty of Medicine and Dentistry, 2Muscular Dystrophy Canada Research Chair, Department of Medical Genetics,University of Alberta Faculty of Medicine and Dentistry

Chapters

  • 00:05Title
  • 01:00LNA/DNA Mixmer Transfection Followed by One-step RT-PCR to Measure the Exon Inclusion Rate of SMN2
  • 05:07Results: Use of LNA/DNA Mixmers to Study Efficient Inclusion of SMN2 Exon and Rescue of SMN Protein Expression
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

Forskellige antisense oligonukleotider (AONs) har vist sig at fremkalde exon integration (splice modulation) og redde SMN udtryk for spinal muskelatrofi (SMA). Her, beskriver vi en protokol for AON lipotransfection til at fremkalde exon integration i SMN2 gen og evalueringsmetoder for at fastlægge effekten i SMA patient fibroblaster.

Tags

Keywords: Exon InclusionSplice SwitchingAntisense OligonucleotidesSMAFibroblastsLipotransfectionTransfectionLNA/DNA MixmerSerum-deprived MediaTransfection ReagentCell ConfluencyGPC ReagentRNA Extraction

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57530?article_type=v

Vascular Occlusion Training for Inclusion Body Myositis: A Novel Therapeutic Approach
check_url/57530?article_type=v

Eksperimentel Generering af Carcinoma-associerede fibroblaster (cafeer) fra Human brystkirtler fibroblaster
check_url/57530?article_type=v

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining
check_url/57530?article_type=v

Cardiac Stress Test induceret af Dobutamin og overvåges af hjertekateterisation i mus
check_url/57530?article_type=v

Evaluering af lungemetastase i musebrysttumor modeller af kvantitativ real-time PCR
check_url/57530?article_type=v

Multi-exon Skipping Brug Cocktail antisenseoligonukleotider i Canine X-bundet Muskelsvindfonden
check_url/57530?article_type=v

En Model af glaukom induceret af Circumlimbal sutur i rotter og mus
check_url/57530?article_type=v

Kvantitativ [18F]-NAF-Pet-MRI-analyse til vurdering af dynamisk knogleomsætning hos en patient med Facetogen lændesmerter
check_url/57530?article_type=v

Invasiv Hæodynamisk vurdering for det højre ventrikel system og hypoxia-induceret pulmonal arteriel hypertension i mus
check_url/57530?article_type=v

Karakteristik Exon Spring effektivitet i DMD patientprøver i kliniske forsøg med antisense oligonukleotider

Read Article