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Användningen av ultraljud guidade vävnad-regisserad cellulära Implantation för etableringen av biologiskt relevanta metastaserande tumör xenograft
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1Departments of Surgery, C.S Mott Children’s and Women’s Hospital,The University of Michigan Medical School, 2Departments of Pathology, C.S Mott Children’s and Women’s Hospital,The University of Michigan Medical School, 3Unit for Laboratory Animal Medicine,The University of Michigan Medical School, 4Departments of Radiology, C.S Mott Children’s and Women’s Hospital,The University of Michigan Medical School, 5Departments of Pediatrics, C.S Mott Children’s and Women’s Hospital,The University of Michigan Medical School
Chapters
- 00:04Title
- 01:13Preparation of Primary Patient-derived Tumor Cells
- 03:29Ultrasound Guided Implantation
- 05:48Results: In Vivo Ultrasound Imaging of Tumor Engraftment and Growth
- 07:22Conclusion
Här presenterar vi ett protokoll för att använda ultraljud-guidad injektion av neuroblastom (NB) och Ewings sarkom (ES) celler (etablerade cellinjer och patientderiverade tumörceller) på biologiskt relevanta platser för att skapa tillförlitliga prekliniska modeller för cancer forskning.
